A Specific Hepatic Transfer RNA for Phosphoserine

A specific hepatic transfer RNA for phosphoserine.

Radioactive O-phosphoryl-L-serine was detected after alkaline deacylation of rat and rooster liver [(3)H]seryl-tRNA acylated in vitro with homologous synthetases. Ribonuclease treatment of this tRNA yielded a compound with the properties of phosphoseryl-adenosine. Benzoylated DEAE-cellulose chromatography of seryl-tRNA yielded four distinct peaks, only one of which contained phosphoserine. A un...

SPBP is a phosphoserine-specific repressor of estrogen receptor alpha.

Multiple signaling pathways stimulate the activity of estrogen receptor alpha (ERalpha) by direct phosphorylation within its N-terminal activation function 1 (AF1). How phosphorylation affects AF1 activity remains poorly understood. We performed a phage display screen for human proteins that are exclusively recruited to the phosphorylated form of AF1 and found the stromelysin-1 platelet-derived...

A reversible oxidative in activation of specific transfer RNA species.

6 Lehrer, S. S., and G. D. Fasman, Biopolymers, 2, 199 (1964). 7Magnasco, V., G. Gray, and C. Nicora, Nuovo Cimento, 34, 1263 (1964). 8 Natta, G., and P. Corradini, Makromol. Chem., 16, 77 (1955); Nuovo Cimento, Suppl., 15, 68 (1960). 9 Corradini, P., and P. Ganis, Nuovo Cimento, Suppl., 15, 104 (1960). 10 Powell, J., Computer J., 7, 155 (1964). 11 Corey, R. B., and L. Pauling, Proc. Roy. Soc. ...

Bcr-abl Silencing by Specific Small-Interference RNA Expression Vector as a Potential Treatment for Chronic Myeloid Leukemia

Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...

A Modified Orcinol Reaction for RNA Determination